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Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.
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Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.
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Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.
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Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.
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Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.
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Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Intestinal Polyamine Metabolism and Mucosal Barrier in Ningxiang and DLY Piglets: Differential Responses to ETEC Challenge

doi: 10.3390/ani16091336

Figure Lengend Snippet: Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h at room temperature (RT), washed in TBST buffer, and incubated overnight with primary antibodies at 4 °C (ODC1, 1:800, cat. #bs-1294R, Bioss, Beijing, China; occludin, 1:1000, cat. #27260-1-AP, Proteintech, Inc., Chicago, IL, USA), followed by secondary antibodies (cat. #A21020, anti-rabbit, 1 μg/L, Proteintech, Inc., Chicago, IL, USA).

Techniques: Concentration Assay, Expressing, Activity Assay